Plasmid Distribution and Evidence for a Proteinase Plasmid in Streptococcus lactis C21
نویسندگان
چکیده
منابع مشابه
Plasmid distribution and evidence for a proteinase plasmid in Streptococcus lactis C2-1.
Five plasmids, distinguishable by their molecular weights (10(6), 2 x 10(6), 5 x 10(6), 10(7), 3 x 10(7), respectively) were isolated from Streptococcus lactis C2. A spontaneous proteinase-negative derivative of this strain lacked the 10(7) plasmid.
متن کاملPlasmids in Streptococcus lactis: evidence that lactose metabolism and proteinase activity are plasmid linked.
Populations of lactose positive (Lac+) and proteinase positive (Prt+) cells from Streptococcus lactis M18, C10, and ML3 grown at 39 degrees C gave rise to increasing proportions of Lac- Prt- clones. The deficiencies did not appear until after a number of generations at the elevated temperature, and the rate depended on the strain.Lac- Prt+ and Lac+ Prt- mutants were isolated after treatment wit...
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A lactose-negative (Lac-), proteinase-negative (Prt-) mutant, designated C145 was isolated from Streptococcus lactis C2 after treatment with nitrosoguanidine and ultraviolet irradiation. The mutant appeared to be cured of the prophage(s) present in S. lactis C2 based on non-inducibility by ultraviolet irradiation or mitomycin C. When cleared lysate material from C145 was subjected, to cesium ch...
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Using Streptococcus thermophilus phages, plasmid transduction in Lactococcus lactis was demonstrated. The transduction frequencies were 4 orders of magnitude lower in L. lactis than in S. thermophilus. These results are the first evidence that there is phage-mediated direct transfer of DNA from S. thermophilus to L. lactis. The implications of these results for phage evolution are discussed.
متن کاملTransformation of Streptococcus lactis Protoplasts by Plasmid DNA.
Polyethylene glycol-treated protoplasts prepared from Streptococcus lactis LM3302, a lactose-negative (Lac) derivative of S. lactis ML3, were transformed to lactose-fermenting ability by a transductionally shortened plasmid (pLM2103) coding for lactose utilization.
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1975
ISSN: 0003-6919
DOI: 10.1128/aem.29.4.546-548.1975